Description
Intended Uses: For the quantitative detection of Human Gasdermin-A (GSDMA) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GSDMA in Human serum, plasma. An antibody specific for GSDMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSDMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSDMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSDMA bound in the initial step. The color development is stopped and the intensity of the color is measured.